A method to profile phosphate containing endogenous metabolites

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steroids drugs The capability of the QqQLit hybrid triple quadrupole linear ion trap mass spectrometer to profile endogenous metabolites has been assessed by the analysis of three different families of metabolites; nucleotides from bacteria and N acyl ethanolamines and N acyl glycerols from rat tissues.Mass spectrometry methods were developed based on employing a survey scan, either precursor ion or neutral loss, coupled with full product ion spectra. This approach identified families of metabolites with a common structural core and provided the structural information for the reliable identification of known and unknown metabolites. By targeting structural similarities, this approach has opened the window of metabolites that can be profiled beyond the constraints of available references standards.A method to profile phosphate containing endogenous metabolites, particularly nucleotide metabolites steroids, was based on the identification of the phosphate moiety following collision induced dissociation. steroids drugs

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